SCSB Crystallography Announcements

August 2007: Through a partnership with the Departments of Biochemistry and Molecular Biology , and Neuroscience and Cell Biology, the Sealy Center for Structural Biology and Molecular Biophysics (SCSB) has recently completed an expansion of the X-Ray facilities. In addition to the purchase of XENOCS confocal optics for data collection, SCSB has purchased the Rigaku CrystalMation system to facilitate high-throughput crystallization screening. The platform technology contains three inter-connected robotics systems: The PHOENIX, the Alchemist II, and the Desktop MINSTRAL, which are integrated through the CrystalTrak crystallization database software program. This technology provides SCSB members and collaborators with automation at each step, from custom screen preparation to crystallization trial imaging and analysis. The system was installed in mid August and has seen heavy use since.

January 2007: New SCSB web site launced. The Entire SCSB web site has been revamped. On-line scheduling is now available for all Instruments.


PUBLICATIONS
These are Structural publications from the Sealy Center for Structural Biology and Molecular Biophysics X-ray Crystallography Laboratory faculty and staff. 
Last Update: July, 2009.

2009

PubMed 2009
Tsalkova T, Blumenthal DK, Mei FC, White MA, Cheng X. Mechanism of EPAC activation: Structural and functional analyses of EPAC2 hinge mutants with constitutive and reduced activities. J Biol Chem. [Epub-2009 Jun 24]. Biophys J. 2009; 963);1083-90 EPUB: {PM} {PDF}
Fuson KL, Ma L, Sutton RB, Oberhauser AF, The c2 domains of human synaptotagmin 1 have distinct mechanical properties, Biophys J. 2009; 963);1083-90 EPUB: {PM} {PDF}

2008

PubMed 2008
Mark Andrew White, Natalia Mast, Ingemar Bjorkhem, Eric F. Johnson, C. David Stout, and Irina A. Pikuleva, The Use of Complementary Cation and Anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1, Acta Cryst. D 2008; 65(16);487-95 EPUB: April 20. {PM} {PDF}
Natalia Mast, Mark Andrew White, Ingemar Bjorkhem, Eric F. Johnson, C. David Stout, and Irina A. Pikuleva, Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principle cholesterol hydroxylase in the brain, PNAS 2008; 105(28);9546-9551 EPUB: July 9. {PM} {PDF}

2007

PubMed 2007
Fuson, K.L., Montes, M., Robert, J. J., Sutton R. B., Structure of Human Synaptotagmin 1 C2A-C2B in the Absence of Ca2+ Reveals a Novel Domain Association, Biochemistry 2008; 46(45);13041-13048 EPUB: Oct. 23. {PM} {PDF}
Zhao Y, Sun L, Muralidhara BK, Kumar S, White MA, Stout CD, Halpert JR., Structural and Thermodynamic Consequences of 1-(4-Chlorophenyl)imidazole Binding to Cytochrome P450 2B4. Biochemistry 2007 Oct 16;46(41):11559-67. EPUB: Sept 22. {PM} {PDF}

2006

PubMed 2006
Aditya Hindupur, Deqian Liu, Yonghong Zhao, Henry D. Bellamy, Mark A. White, and Robert O. Fox, The crystal structure of the E. coli stress protein YciF. Protein Science 2006 EPUB: Sept 25. {PM} {PDF}
Andrew T. Russo, Mark A. White, and Stanley J. Watowich, The Crystal Structure of the Venezuelan Equine Encephalitis Alphavirus nsP2 Protease. Structure 2006 14: 1449-1458. {PM} {PDF}
Montes M, Fuson KL, Sutton RB, Robert JJ. Purification, crystallization and X-ray diffraction analysis of human synaptotagmin 1 C2A-C2B. Acta Cryst. F 62, Page 926-9, Aug 2006. {PM} {PDF}
Singh R, White MA, Ramana KV, Petrash JM, Watowich SJ, Bhatnagar A, Srivastava SK., Structure of a glutathione conjugate bound to the active site of aldose reductase. Proteins: Structure, Function, and Bioinformatics 2006, July 1, 64(1), 101-110; [April 25, Epub ahead of print] {PM} {PDF}
Mukherjee M, Dutta K, White MA, Cowburn D, Fox RO., NMR solution structure and backbone dynamics of domain III of the E protein of tick-borne Langat flavivirus suggests a potential site for molecular recognition. Protein Science 2006, June 15(6), 1342-55. {PM} {PDF}
Russo AT, Watowich SJ, Purification, crystallization and X-ray diffraction analysis of the C-terminal protease domain of Venezuelan equine encephalitis virus nsP2. Acta Cryst. F 62(6), Page 514-7, June 2006. {PM} {PDF}
Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD., Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: Insight into P450 conformational plasticity and membrane interaction. J Biol Chem. 2006, March 3, 281(9), 5973-81; [Epub ahead of print: Dec 21] {PM} {PDF}
Sutton RB, Vishnivetskiy SA, Robert J, Hanson SM, Raman D, Knox BE, Kono M, Navarro J, Gurevich VV., Crystal structure of cone arrestin at 2.3A: evolution of receptor specificity., J Mol Biol. 2005 Dec 16;354(5):1069-80. Epub 2005 Nov 2. {PM} {PDF}

2005

PubMed 2005
Schultz DA, Friedman AM, White MA, Fox RO., The crystal structure of the cis-proline to glycine variant (P114G) of ribonuclease A, Protein Sci. 2005 Nov; 14(11):2862-70. [Epub ahead of print; Sep 30] {PM} {PDF}
Bi-Hung Peng, Mark A. White, Gerald A. Campbell, Jebamony J. Robert, J. Ching Lee, Roger B. Sutton, Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin Acta Cryst. F 61, Page 369-371, Apr 2005. {PM} {PDF}
Czerwinski EW, Midoro-Horiuti T, White MA, Brooks EG, Goldblum RM., Crystal structure of Jun a 1, the major cedar pollen allergen from Juniperus ashei, reveals a parallel beta-helical core. J. Biol. Chem 280, 5, 3740-6, 2005. {PM} {PDF}

2004

PubMed 2004
Liu D, Zhao Y, Fan X, Sun Y, Fox RO., Escherichia coli stress protein YciF: expression, crystallization and preliminary crystallographic analysis. Acta Cryst. D 60, Page 2389-90, Dec. 2004. {PM} {PDF}
Emily E. Scott, Mark A. White, You Ai He, Eric F. Johnson, C. David Stout, and James R. Halpert, Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl)imidazole at 1.9 A resolution: Insight into the range of P450 conformations and coordination of redox partner binding J. Biol. Chem 279, 26, 27294-301, 2004. {PM} {PDF}
Liu D, Zhao Y, Fan X, Sun Y, Fox RO, Expression, crystallization and preliminary crystallographic analysis of YciE, a stress protein from Escherichia coli. Acta Cryst. D 60, Page 1888-9, Oct. 2004. {PM} {PDF}

2003

PubMed 2003
Yonghong Zhao, Deqian Liu, Warna D. Kaluarachi, Henry D. Beleamy, Mark A. White, and Robert O. Fox, (2003), The crystal structure of Escherichia coli heat shock protein YedU reveals three potential catalytic active sites, Protein Science 12, 2303-11, 2003. {PM} {PDF}
Emily E. Scott, You Ai He, Michael R. Webster, Mark A. White, Christopher C. Chin, James R. Halpert, Erik F. Johnson, and C. David Stout, (2003), An open conformation of mammalian cytochrome P450 2B4 at 1.6-A resolution, PNAS 100, 13196-13201, 2003. {PM} {PDF}
Mark A. White, Deqian Liu, Michael R. Holbrook, Robert E. Shope, Alan D. T. Barrett and Robert O. Fox, (2003), Crystallization and preliminary X-ray diffraction analysis of Langat virus envelope protein domain III, Acta Cryst. D 59, 1049-51, 2003. {IUCr} {PDF}
Deqian Liu, Terumi Midoro-Horiuti, Mark A. White, Edward G. Brooks, Randall M. Goldblum and Edmund W. Czerwinski (2003), Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei, Acta Cryst. D 59, 1052-53, 2003. {IUCr} {PDF}
Landau EM, Pebay-Peyroula E, Neutze R. Structural and mechanistic insight from high resolution structures of archaeal rhodopsins. FEBS Lett. 555(1), Page 51-6. Nov 27, 2003. {PM} {PDF}

2002

PubMed 2002

2001

PubMed 2001
John O. Wooll, Robert H. E. Friesen, Mark A. White, Stanley J. Watowich, Robert O. Fox, J. Ching Lee* and Edmund W. Czerwinski*, (2001), Structural and Functional Linkages Between SubunitInterfaces in Mammalian Pyruvate Kinase, J. Mol. Biol. 312, 525-540, 2001. {PM} {PDF}

2000

PubMed 2000

1999

PubMed 1999
Mark A. White, Stanley J. Watowich, and Robert O. Fox, (1999), Calibration of Spiral Readout Image Plate Detectors, J. Appl. Cryst. 32, 65-70, 1999. {IUCr} {PDF}

Calendar of Crystallographic Meetings

Jan. 24-25, 2007 Advances in Protein Crystallography: San Francisco, CA. (info)
Feb. 28- Mar. 2, 2007 SPINE2/ BIOXHIT Crystallization Workshop: EMBL-Hamburg Outstation, Germany. (info)
March 3-7th, 2007 Biophysical Society: Baltimore, MD. (info)
March 22-25th, 2006 Winter school on Soft X-rays in Macromolecular Crystallography: Seefeld, Austria. (info)
March 27, 2009 SCSB Structural Biology Symposium: , Galveston, TX (info)
March 30-Apr 1, 2007 Texas Protein Folders: Camp Allen, TX. (info)
April 17-19, 2007 The British Crystallographic Association spring meeting:Canterbury, UK. (info)
April 4-7, 2006 The X6A workbench: Advanced Structural Biology Tools: Brookhaven, NY. (info)
April 11-15, 2007 York MM Workshop: York, UK (info)
April 19-24, 2009 RapiData 2008 Tutorial Course: BNL, LI ,NY (info)
May 25-26, 2006 Modern Drug Target Crystallography and Structure Based Drug Discovery: Molsoft LLC facility, San Diego, California (info)
May 15-20, 2006 EMBO Practical Course: "Structural Characterization of Macromolecular Complexes", Grenoble, (info)
June 7-17, 2007 SB of Large Macromolecular Assemblies: Erice, Italy. (info)
July 13-18, 2008 Gordon Conference on Crystallographic Techniques: Boston, MA (info)
July 21-26, 2007 ACA: Salt Lake City, Utah. (info)
July 21-25, 2007 Protein Society: Boston (info)
Aug. 6-11, 2006 ECM-23: European Crystallography Meeting: Leuven, Belgium. (info)
Sept. 9-15, 2007 The 14th Summer School in Protein Crystallography: St. Andrews, Scotland. (info)
Sept. 15-16, 2006 5th International NCCR Symposium on New Trends in Structural Biolog: ETH Zurich, Switzerland. (info)
Sep 29-Oct 3, 2008 Como 10th Int. School on the Crystallography of Biological Macromolecules: Como, Italy (info)
Oct. 6-13, 2006 BIOCRYS 2006: Fundamentals of Modern Methods in Biocrystallography: Oeiras, Portugal. (info)
Oct. 19-26, 2006 4th. INTERNATIONAL CONFERENCE ON STRUCTURAL GENOMICS: Tokyo/Beijing. (info)
Oct. 25-26, 2007 17th Annual SouthWest Macromolecular Symposium: Woodlands, TX. (info)


X-ray User's Group Newsletters

XUG_2009 #1 (Oct)
XUG_2005_Jan XUG_2006_Feb
XUG_2004_May XUG_2004_Oct XUG_2005_Jan_16
XUG_2004_Jul XUG_2004_July XUG_2004_Jun
XUG_2004_April XUG_2004_Aug XUG_2004_Dec

Keck Seminar Announcements Archive

(See the SCSB EVENTS Calendar for a listing of current Seminar.)
2008
Jan11 M. Pettitt Jan18 Susan Amara Jan25 Winners-pt1
Feb01 Jennifer West Feb15 Winners-pt2 Feb22 Jonas S. Almeida
Feb29 Robert Powers Mar15 Werner Braun Mar28 Xiaodong Zhang
Apr04 Steven J. Cox Apr11 Jin Wang Apr18 Eva M Sevick
Fall
Sep05 John N. Weinstein Sep12 Michelle Malizia Sep19 Yuriy Fofanov
Sep26 Glen Legge Oct03 GCC/Keck Annual Research Conference Oct10 Devika Subramanian
Oct17 E. Lynn Zechiedrich Oct24 Manolis Doxastakis Oct31 John Spudich
Nov07 Farshid Guilak Nov14 John Tyson Nov21 Renata Pasqualini
Dec05 Kyriacos A. Athanasiou
2007
Nov30 David Gorenstein Dec07 Kevin Ridge
Nov02 Richard Brennan Nov09 Mehmet Sarikaya Nov16 Ariel Fernandez
Sep28 Brian P. Zambrowicz Oct05 Paul Rothemund Oct26 Neal Pellis
Sep07 Timothy Palzkill Sep14 Chengzhi Cai Sep21 Yuhai Tu
KECK_2007-04-20
KECK_2007-03-23 KECK_2007-03-30 KECK_2007-04-13
KECK_2007-02-23 KECK_2007-03-02 KECK_2007-03-16
KECK_2007-02-02 KECK_2007-02-09 KECK_2007-02-16
KECK_2007-01-12 KECK_2007-01-19 KECK_2007-01-26
2006
KECK_2006-12-01 KECK_2006-11-17 KECK_2006-11-03
KECK_2006-10-27 KECK_2006-10-06 KECK_2006-09-29
KECK_2006-09-22 KECK_2006-09-15 KECK_2006-09-08
KECK_2006-08-25 KECK_2006-05-05 KECK_2006-04-28
KECK_2006-04-21 KECK_2006-04-07 KECK_2006-03-31
KECK_2006-03-24 KECK_2006-03-10 KECK_2006-03-03
KECK_2006-02-24 KECK_2006-02-17 KECK_2006-02-10
KECK_2006-02-03 KECK_2006-01-27 KECK_2006-01-20
KECK_2006-01-13
2005
KECK_2005-12-02 KECK_2005-11-18 KECK_2005-11-04
KECK_2005-10-19 KECK_2005-10-14 KECK_2005-09-30
KECK_2005-09-23 KECK_2005-09-16 KECK_2005-09-09
KECK_2005_08_26 KECK_2005_04_22 KECK_2005_04_15
KECK_2005_04_01 KECK_2005_03_18 KECK_2005_03_04
KECK_2005_02_25 KECK_2005_02_18 KECK_2005_02_11
KECK_2005_02_04 KECK_2005_01_28 KECK_2005_01_21
KECK_2005_01_14
2004
KECK_2004_12_05 KECK_2004_11_19 KECK_2004_11_12
KECK_2004_11_05 KECK_2004_10_29 KECK_2004_10_22
KECK_2004_10_15 KECK_2004_10_01 KECK_2004_09_17
KECK_2004_09_10 KECK_2004_09_03 KECK_2004_04_16
KECK_2004_03_26 KECK_2004_02_27 KECK_2004_02_20
KECK_2004_02_13 KECK_2004_02_06 KECK_2004_01_23
KECK_2004_01_16
2003
KECK_2003_12_05 KECK_2003_12_03 KECK_2003_11_14

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